A full complement of transfer RNA genes in the Candida albicans genome
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چکیده
The functions of non-coding genes, such as transfer RNA (tRNA) genes, in Candida albicans have been largely overlooked, though it is widely realized that they play important roles in regulating gene expression. As an initial effort to study small RNA genes from C. albicans, we have identified a total of 132 tRNA genes from the nuclear genome using tRNAscan-SE. These tRNA genes represent 43 unique tRNA gene species decoding all of the 20 amino acids. Together with the previously annotated 30 tRNA genes, of which 24 are unique, from the mitochondrial genome of C. albicans, a total of 162 tRNA genes were used in this study. Since the "universal" leucine codon CTG specially encodes for serine in C. albicans, this non-standard translational event may add some special features to the tRNA genes. Phylogenetic relationships of tRNA genes from the nuclear and mitochondrial genomes were compared to demonstrate some recent gene duplications and interorganellar gene transfer. The correlation of codon usage with copy numbers of the tRNA genes was also discussed.
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تاریخ انتشار 2007